Comparative evaluation of the effect of antioxidants on the chilled-stored ram semen

The aim of this investigation was to assess the effect of different antioxidants on the motility andmembrane integrity of the ram semen during storage at 5ºC for 0, 12, 24, 48 and 72 hrs after collection anddilution. A total of 57 ejaculates from 12 Bakhtiary rams was collected by artificial vagina and were extended1 : 3 with egg yolk-citrate buffer, which was containing one of the following antioxidants: BSA 1% (w/v),BSA 3%, BSA 1% + sucrose 10%, BSA 3% + sucrose 10%, vitamin E (1 mg), vitamin E (2 mg), ascorbicacid (0.9 mg/ml) and control group without any antioxidant. Percent of motile sperm (PMS), rate of forwardmovement (RFM) and membrane integrity in extenders contained vitamin E groups were significantly higherup to 48 hrs than other groups (P